Photocrosslinking of G‐Quadruplex‐Forming Sequences found in Human Promoters

Jillian E. Smith-Carpenter; John-Stephen Taylor

doi:10.1111/php.12991

Photocrosslinking of G‐Quadruplex‐Forming Sequences found in Human Promoters

Jillian E. Smith-Carpenter, John-Stephen Taylor

Fairfield University

Research output: Contribution to journal › Article › peer-review

Abstract

While is it well known that human telomeric DNA sequences can adopt G&hyphen;quadruplex structures, some promoters sequences have also been found to form G&hyphen;quadruplexes, and over 40% of promoters contain putative G&hyphen;quadruplex&hyphen;forming sequences. Because UV light has been shown to crosslink human telomeric G&hyphen;quadruplexes by cyclobutane pyrimidine dimer (CPD) formation between T's on adjacent loops, UV light might also be able to photocrosslink G&hyphen;quadruplexes in promoters. To investigate this possibility, 15 potentially UV&hyphen;crosslinkable G&hyphen;quadruplex&hyphen;forming sequences found in a search of human DNA promoters were UVB irradiated in vitro , and three were confirmed to have formed nonadjacent CPDs by mass spectrometry. In addition to nonadjacent T=T CPDs found in human telomeric DNA, a nonadjacent T=U CPD was discovered that presumably arose from deamination of a nonadjacent T=C CPD. Analysis of the three sequences by circular dichroism, melting temperature analysis and chemical footprinting confirmed the presence of G&hyphen;quadruplexes that could explain the formation of the nonadjacent CPDs. The formation of nonadjacent CPDs from the sequences in vitro suggests that they might be useful probes for the presence of non&hyphen;B DNA structures, such as G&hyphen;quadruplexes, in vivo , and if they were to form in vivo, might also have significant biological consequences.

Original language	American English
Journal	Photochemistry and photobiology
Volume	95
DOIs	https://doi.org/10.1111/php.12991
State	Published - Jan 1 2019

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Chemistry
Physical Sciences and Mathematics

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10.1111/php.12991License: CC BY

https://onlinelibrary.wiley.com/doi/full/10.1111/php.12991

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@article{040dd84e3b8645be9a6186ab3f82aa19,

title = "Photocrosslinking of G‐Quadruplex‐Forming Sequences found in Human Promoters",

abstract = " While is it well known that human telomeric DNA sequences can adopt G\&hyphen;quadruplex structures, some promoters sequences have also been found to form G\&hyphen;quadruplexes, and over 40\% of promoters contain putative G\&hyphen;quadruplex\&hyphen;forming sequences. Because UV light has been shown to crosslink human telomeric G\&hyphen;quadruplexes by cyclobutane pyrimidine dimer (CPD) formation between T's on adjacent loops, UV light might also be able to photocrosslink G\&hyphen;quadruplexes in promoters. To investigate this possibility, 15 potentially UV\&hyphen;crosslinkable G\&hyphen;quadruplex\&hyphen;forming sequences found in a search of human DNA promoters were UVB irradiated in vitro , and three were confirmed to have formed nonadjacent CPDs by mass spectrometry. In addition to nonadjacent T=T CPDs found in human telomeric DNA, a nonadjacent T=U CPD was discovered that presumably arose from deamination of a nonadjacent T=C CPD. Analysis of the three sequences by circular dichroism, melting temperature analysis and chemical footprinting confirmed the presence of G\&hyphen;quadruplexes that could explain the formation of the nonadjacent CPDs. The formation of nonadjacent CPDs from the sequences in vitro suggests that they might be useful probes for the presence of non\&hyphen;B DNA structures, such as G\&hyphen;quadruplexes, in vivo , and if they were to form in vivo, might also have significant biological consequences.",

author = "Smith-Carpenter, \{Jillian E.\} and John-Stephen Taylor",

year = "2019",

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day = "1",

doi = "10.1111/php.12991",

language = "American English",

volume = "95",

journal = "Photochemistry and photobiology",

}

TY - JOUR

T1 - Photocrosslinking of G‐Quadruplex‐Forming Sequences found in Human Promoters

AU - Smith-Carpenter, Jillian E.

AU - Taylor, John-Stephen

PY - 2019/1/1

Y1 - 2019/1/1

N2 - While is it well known that human telomeric DNA sequences can adopt G&hyphen;quadruplex structures, some promoters sequences have also been found to form G&hyphen;quadruplexes, and over 40% of promoters contain putative G&hyphen;quadruplex&hyphen;forming sequences. Because UV light has been shown to crosslink human telomeric G&hyphen;quadruplexes by cyclobutane pyrimidine dimer (CPD) formation between T's on adjacent loops, UV light might also be able to photocrosslink G&hyphen;quadruplexes in promoters. To investigate this possibility, 15 potentially UV&hyphen;crosslinkable G&hyphen;quadruplex&hyphen;forming sequences found in a search of human DNA promoters were UVB irradiated in vitro , and three were confirmed to have formed nonadjacent CPDs by mass spectrometry. In addition to nonadjacent T=T CPDs found in human telomeric DNA, a nonadjacent T=U CPD was discovered that presumably arose from deamination of a nonadjacent T=C CPD. Analysis of the three sequences by circular dichroism, melting temperature analysis and chemical footprinting confirmed the presence of G&hyphen;quadruplexes that could explain the formation of the nonadjacent CPDs. The formation of nonadjacent CPDs from the sequences in vitro suggests that they might be useful probes for the presence of non&hyphen;B DNA structures, such as G&hyphen;quadruplexes, in vivo , and if they were to form in vivo, might also have significant biological consequences.

AB - While is it well known that human telomeric DNA sequences can adopt G&hyphen;quadruplex structures, some promoters sequences have also been found to form G&hyphen;quadruplexes, and over 40% of promoters contain putative G&hyphen;quadruplex&hyphen;forming sequences. Because UV light has been shown to crosslink human telomeric G&hyphen;quadruplexes by cyclobutane pyrimidine dimer (CPD) formation between T's on adjacent loops, UV light might also be able to photocrosslink G&hyphen;quadruplexes in promoters. To investigate this possibility, 15 potentially UV&hyphen;crosslinkable G&hyphen;quadruplex&hyphen;forming sequences found in a search of human DNA promoters were UVB irradiated in vitro , and three were confirmed to have formed nonadjacent CPDs by mass spectrometry. In addition to nonadjacent T=T CPDs found in human telomeric DNA, a nonadjacent T=U CPD was discovered that presumably arose from deamination of a nonadjacent T=C CPD. Analysis of the three sequences by circular dichroism, melting temperature analysis and chemical footprinting confirmed the presence of G&hyphen;quadruplexes that could explain the formation of the nonadjacent CPDs. The formation of nonadjacent CPDs from the sequences in vitro suggests that they might be useful probes for the presence of non&hyphen;B DNA structures, such as G&hyphen;quadruplexes, in vivo , and if they were to form in vivo, might also have significant biological consequences.

UR - https://digitalcommons.fairfield.edu/chemistry-facultypubs/43

UR - https://onlinelibrary.wiley.com/doi/full/10.1111/php.12991

U2 - 10.1111/php.12991

DO - 10.1111/php.12991

M3 - Article

VL - 95

JO - Photochemistry and photobiology

JF - Photochemistry and photobiology

ER -

Photocrosslinking of G‐Quadruplex‐Forming Sequences found in Human Promoters

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